LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2057..2153) /label=attL2 /note="attL2 (c)" misc_signal complement(2168..2187) /note="T7 Promoter/priming site (c)" primer_bind 2195..2211 /note="M13 Reverse priming site" gene 2324..3133 /note="Kanamycin resistance gene" rep_origin 3254..3927 /note="pUC origin" vector join(2065..3930,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2050 /dnas_title="CHST7-Nterm-TEVfusion-pDONR221" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /sex="male" /tissue_type="brain" /dev_stage="adult" CDS 692..2050 /dnas_title="N-acetylglucosamine-6-O-sulfotransferase" /codon_start=1 /product="N-acetylglucosamine-6-O-sulfotransferase" /protein_id="BAB13770.1" /db_xref="GI:10336539" /translation="MKGRRRRRREYCKFALLLVLYTLVLLLVPSVLDGGRDGDKGAEH CPGLQRSLGVWSLEAAAAGEREQGAEARAAEEGGANQSPRFPSNLSGAVGEAVSREKQ HIYVHATWRTGSSFLGELFNQHPDVFYLYEPMWHLWQALYPGDAESLQGALRDMLRSL FRCDFSVLRLYAPPGDPAARAPDTANLTTAALFRWRTNKVICSPPLCPGAPRARAEVG LVEDTACERSCPPVAIRALEAECRKYPVVVIKDVRLLDLGVLVPLLRDPGLNLKVVQL FRDPRAVHNSRLKSRQGLLRESIQVLRTRQRGDRFHRVLLAHGVGARPGGQSRALPAA PRADFFLTGALEVICEAWLRDLLFARGAPAWLRRRYLRLRYEDLVRQPRAQLRRLLRF SGLRALAALDAFALNMTRGAAYGADRPFHLSARDAREAVHAWRERLSREQVRQVEAAC APAMRLLAYPRSGEEGDAEQPREGETPLEMDADGAT" misc_feature 692..2050 /note="CHST7 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cggcggccgc gacggggaca agggcgccga 721 gcactgcccc ggcctgcagc gcagcctggg agtgtggagc ctggaggcgg cggcggccgg 781 cgaacgcgag cagggagcgg aggcgcgggc cgccgaggaa gggggcgcga accagtctcc 841 tcggttccca agcaacctca gcggcgctgt cggggaggca gtgtctcgcg agaagcagca 901 catctacgtg catgccacct ggcgcaccgg ctcgtccttc ctgggcgaac tctttaacca 961 gcacccggac gttttctact tgtatgagcc catgtggcat ctatggcagg cgctgtatcc 1021 gggcgacgcc gagagcttgc agggcgcgct gcgcgacatg ctgcgttcgc tcttccgctg 1081 cgacttctcc gtgctgcggc tgtacgcgcc gccgggggac cccgctgcgc gcgccccgga 1141 cacggccaat cttaccacgg ccgccctctt ccgctggcgg actaacaagg tcatctgctc 1201 gccgccactg tgtcctggcg caccccgtgc ccgggccgag gtgggcctcg tcgaggacac 1261 cgcctgcgag cgcagctgcc cacccgtggc gatacgcgcc ctggaggccg agtgccgaaa 1321 gtacccggtg gtggtcatca aggacgtgcg cctgctcgat ctgggcgtgc tggtgcccct 1381 gttgcgtgat ccaggcctca acctgaaggt ggtgcagctt ttccgcgacc cgagggcggt 1441 gcacaactcg cgcctcaagt ctaggcaggg actgctgcgc gagagcatcc aggtgctgcg 1501 cacccgccag aggggcgacc gcttccaccg tgtgctgctg gcgcacggcg tgggtgctcg 1561 ccccgggggc cagtctcgcg cgctgcccgc cgcgccgcgc gccgatttct tcctgaccgg 1621 tgcgctcgag gtgatctgcg aagcctggct gcgcgatctg cttttcgcgc gcggcgcgcc 1681 cgcctggctg cggcgccgct acctgaggct gcgctatgag gacctggtgc ggcagccacg 1741 cgcccagctg cgccgcctgc tgcgcttctc cgggctacgc gcgctcgcag cgctcgatgc 1801 cttcgcgctc aacatgactc gcggcgcggc ctacggcgcc gaccggccct tccacctgtc 1861 agcgcgcgac gcccgggagg cggtgcacgc ctggcgcgag cgcctgagcc gagagcaggt 1921 gcgccaggtg gaggccgcct gcgctccagc catgcgtctg ctcgcctacc ctcgcagcgg 1981 agaggagggc gacgcggagc agcccaggga aggggagacg ccgctggaga tggatgccga 2041 cggcgccacg TAGGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 2101 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 2161 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 2221 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 2281 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 2341 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 2401 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 2461 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 2521 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 2581 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 2641 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 2701 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 2761 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 2821 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 2881 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 2941 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 3001 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 3061 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 3121 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 3181 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 3241 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 3301 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 3361 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3421 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3481 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3541 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3601 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3661 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 3721 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 3781 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 3841 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 3901 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //